CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development of Methods Based on Isotopic Dilution in Connection with LC-MS to Evaluate the Presence of Veterinary Drugs and Related Chemicals in Aquaculture Fish.
Autor/es:
MONFERRÁN, MAGDALENA VICTORIA; AMÉ, MARÍA VALERIA; WUDERLIN, DANIEL ALBERTO
Lugar:
Pretoria
Reunión:
Encuentro; IAEA-FAO. Meeting Pretoria (Sudáfrica); 2018
Institución organizadora:
International Atomic Energy Agency.
Resumen:
The research project aims to produce state of the art methods for the analysis of veterinary drugs, including their metabolites and impurities in edible fish caught at field and from aquaculture system. Screening methods based on high resolution mass spectrometry (Orbitrap and QTOF) were developed for a mixture of 50 antibiotics and relative compounds for veterinary use. Then, three extraction procedures were compared for a group of antibiotics representing different chemical structure families. Variable recoveries (20 to 102%) were obtained from fish tissues, but two methods were selected for further studies depending on the objective of the work. QTOF screening was tested in fish extracts allowing the detection of flumequine in concentrations below the MRL (fish). A confirmatory method for quantification of antibiotics and related compounds based on isotopic dilution, by LC-ESI MS/MS triple quadrupole was developed in IDAEA (Barcelona, Spain) for the same compounds. This method still need to be validated for fish extracts. Fish samples commercially available were collected from three Argentinean aquaculture farms during summer and winter (2017-2018). Also fish samples of Oncorhynchus kisutch (coho or silver salmon) were collected in big supermarket chains and local markets in Valdivia, Chile.Work plan for next year includes further validation of the confirmatory method in Argentina, the measurement of target compounds in Argentinean and Chilean fish samples based on isotopic dilution by LC-ESI MS/MS triple quadrupole, and to start with the analysis of bioaccumulation and biotransformation of flumequine or oxytetracicline in a local aquaculture farm with Piaractus mesopotamicus (pacú). A direct contact with National Service of Agri-Food Health and Quality (SENASA, Argentina) authorities will be held in order to strength the impact of the project in Argentina. Also the participation in proficiency testing will be considered. The studies on pathways of flumequine biotransformation will be performed depending on the availability of 7-OH-flumequine in the network, since this metabolite is not commercially available in Argentina.