Study of mechanisms underlying thermotolerance activity induced by CHLOROGENIC ACID in C elegans

ASIS RAMON; CARRANZA ANDREA

Buenos Aires

Congreso; Joint meeting of bioscience society; 2017

Chlorogenic acid (CGA) is a polyphenol compound present in foods associated to different benefic properties. In previous studies we demonstrated that CGA confers thermal stress resistance in C.elegans exposed to lethal temperature (37ºC). Its role in the stress resistance is not fully understood. Autophagy is thought to promote cell survival by providing fundamental building blocks to maintain energy homeostasis during starvation or stressful situations. On the other hand, hypoxia-inducible-factor (HIF)-1, a master transcriptional regulator of adaptation to hypoxia, plays a central role in stress resistance. In these sense, in the present study we have proposed to investigate the relationship between CGA, HIF-1 and autophagy. Results showed that CGA was unable to confer thermal stress in worms with loss of function of HIF-1 (ZG31) or with autophagy defects (cdc-48.2(tm659)). Moreover, preincubation of N2 worm with CGA before to be expose to thermal stress showed an increment in autophagy flux and HIF-1 activity. Finally, interactions between HIF-1 and autophagy that underlying CGA activity was explore by RNAi assay. Here, we demonstrate that autophagy and HIF-1 are mechanisms necessary for CGA activity. All together, these preliminary results tend to explain the molecular mechanism that CGA activate in C elegans and evidence a possible hormetic response.