Titanium Dioxide Nanoparticles Identification In Fish Cells: Subcellular Internalization

FERNANDES MN; MONFERRAN M.V.; WUNDERLIN DA; ELLIOT M; MATSUMOTO ST; ROCHA L.D; DUARTE ID; MENDES VAS; SOUZA IC

Buzios-Río de Janeiro

Congreso; 6º Congresso Brasileiro de Microscopia; 2017

Among the varieties of NP produced, metal oxides have been increasingly applied due to their photocatalytic nature, particularly, the titanium dioxide NP (TiO2-NP). Considering the presence of TiO2-NP in the environment due industrial and sewage releasing, especially in aquatic ecosystems, in-situ studies are required to evaluate the behaviour of theses NP in the environmental compartments and biota. Therefore, the purpose of this study was to identify the nanocrystallographic structure of TiO2-NP in abiotic matrixes as well in fish cells. Scanning electron microscope coupled with electron backscatter detector (BSE) were used to identify chemical contrast, secondary electrons (SE) were used to identify morphology and X-ray Dispersive Energy Spectroscopy (EDS) were used to identify chemical elements. Nanodiffraction was used for particles size close to 100 nm and lower to identify the crystallographic structure. Electron nanodiffraction was performed to find axes of high symmetry zone in nanoparticles containing Ti and O using the holder TEM double tilt. Nanodiffraction pattern were indexed using Jems software (Electron Microscopy Software, P.Stade Imann, Switzeland) and theoretical crystallography of the ICSD FIZ Karlsruhe, Germany. At least 2 high symmetry zone axes of the same nanoparticle were indexed. Nanocrystallographic analyses showed that TiO2-NP was in rutile phase in samples from atmospheric particulate matter and fish organs suggesting that atmospheric particulate matter may be the source of TiO2-NP in these ecosystems.