CIBICI 14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
STUDY OF N-LINKED GLYCOPROTEINS IN TEARS FROM PATIENTS WITH CLIMATIC DROPLET KERATOPATHY IN ARGENTINA
Autor/es:
SERRA H M; R.W. BEUERMAN,; L. ZHOU,; AI PING CHEW,; SIEW KWAN KOH; CAFARO T A,; URRETS-ZAVALIA EA; URRETS ZAVALIA, JA; LI S
Lugar:
MIAMI
Reunión:
Congreso; ARVO 2009; 2009
Resumen:
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Since glycoproteins are
potentially biomarkers of diseases we decided to investigate N-linked
glycoproteins in tears of CDK patients and compare these profiles with tears
from normal individuals. Methods: 13 CDK patients and 11 control subjects with
clinically normal eyes and no history of eye disease or eye surgery were
recruited for this study.Tear samples were pre-fractionated using a
hydrazide-resin capture method, and the previously N-glycosylated peptides were
then subjected to two-dimensional nano-LC-nano-ESI-MS/MS analysis to obtain
peptide fragmentation patterns for identification through protein database
searches. Quantitative comparisons of N-glycoprotein profiles in tear fluid of
patients with CDK and to that of non-diseased controls used glycopeptide
capture, iTRAQ labeling and 2D nano-LC-nano-ESI-MS/MS analysis.
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The significant
up-regulation of N-glycosylated haptoglobin in CDK, which is related to
exposure to ultraviolet irradiation, could be clinically significant in terms of
disease etiology and biomarker discovery. Further analysis of the biochemical
pathways of significant proteins should improve our understanding of CDK
