MICROBIOTA, IGG AND CD64+ MACROPHAGES: THEIR INTERACTIONS IN THE COLON LAMINA PROPRIA

ROMAGNOLI, PABLO; MACCIÓ MARETTO, LISA; FRANCO NICOLAS NAZAR; CORREA SILVIA GRACIELA; BARRIOS BIBIANA ELISABET

Buenos Aires

Congreso; Reunión conjunta de sociedades de biociencia; 2017

Intestinal macrophages (MΦs) identified by CD11b, F4/80 and CD64 markers play homeostatic roles in the colonic environment. To evaluate frequency and localization of these cells, whole mount colon sections were stained with fluorescent anti-CD64 and anti-CD11b antibodies. As these MΦs can uptake immune complexes (ICs), we used ex vivo and in vitro strategies to evaluate the uptake of IgG-ICs and stimulatory activity during inflammatory conditions. Ex vivo, we obtained colonic lamina propria MΦs from control and colitis mice by enzymatic digestion and cell sorting using the F4/80 marker. MΦs were stimulated 1 h at 37°C with ICs and the internalized IgG was evaluated by Western blot. In vitro, bone marrow-derived MΦs were cultured with conditioned medium obtained out of colonic explant cultures from control or colitis mice; in the later condition a significant increment in Ly6c and F4/80 expression was found (p