Increased mRNA levels of proinflammatory cytokines and TLR/NFKB pathway molecules in response to Chlamydia trachomatis infection in CD45- cells from prostate

MACKERN-OBERTI, JP, BRESER, ML, NUÑEZ N, MOTRICH, RD, MACCIONI M, RIVERO, VE

CHILE

Congreso; 9th Latin American Congress of Imunology (ALAI; 2009

ALAI

Prostatitis is a common disease being Chlamydia trachomatis (CT) one of the common pathogens associated with it. We have previously reported that Primary Prostatic Cell cultures (PPEC) express markers of epithelial cell (cytokeratin in 80%) and leukocyte markers (CD45 in 2-5%) and respond to CT infection with the release of many inflammatory components. In the present work we analyze the response of CD45 negative PPEC and CD45 positive cells to CT infection studying the expression of different Toll-Like Receptor Signaling genes using a PCR Array kit. Prostate CD45 negative and CD45 positive cells were sorted with MACS using anti-CD45 conjugated with magnetic beads. CD45 positive cells infection induced mRNA upregulation of several genes as MCP1(fold up regulation change of 22 times), CSF2(145), CSF3(10800), IP10(217), IL1a(107), IL1b(80), IL1r1(10), IL6(60), IFNb1(116), TNF(14), IL10(20), CD86(22), IRAK2(9), Eif2ak2(20), Ptgs28(190), TLR3(8). Prostatic CD45 negative cells infection of induced the upregultaion of MCP(22), CSF3(13), IP10(14), MAPKK4(4), MAPKK8ipr3(4), Nfkbib(77), Nfrkb(175), Pglyrp1(7), Rela(800), Tbk1(90), TLR3 (13), Tnfaip3(5), Tollip(11). These results demonstrate that   epithelial cells and leukocytes respond in a different way in response to CT infection and suggest that resident non-leukocyte cells may play an important role in local chlamydial infection modulating innate immune response of resident leukocytes.