STARD7: BEYOND THE ROLE OF LIPID TRANSFER PROTEIN

FLORES MARTIN J

Córdoba

Congreso; XXI Jornadas científicas de la Sociedad de Biología de Córdoba; 2017

Sociedad de Biología de Córdoba

Alterationsin the quantity, quality and distribution of intracellular lipids lead to different pathologies such as cardiovascular and neural diseases, metabolic defects, and cancer, among others. The StarD7 protein, identified in our laboratory, belongs to the START domain superfamily of proteins, which participate in transport, metabolism and intracellular lipid signaling. This study was performed to address on the effect of StarD7 siRNA in different epithelial cell lines. StarD7 knockdown in trophoblast cells produced a reduction in ABCG2 lipid/xenobiotic carrier protein and phospholipid synthesis, as well as in cell proliferation and migration. In contrast, morphological and biochemical differentiation markers wereincreased. In addition, StarD7silencing in HepG2 cells produced alterations in endoplasmic reticulum (ER) and mitochondria morphology. Furthermore, ER stress, increased reactive oxygen species and antioxidant enzymeswas observed. Also, adegradation of p53 protein through the proteasome was detected. These results suggest that beyond its role in lipid transport, StarD7 contributes to modulate cellular redox homeostasis