CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF FACTORS SECRETED BY MURINE MELANOMA B16 CELLS ACTIVATED VIA TOLL LIKE RECEPTOR 4 ON THE MATURATION STATE OF DENDRITIC CELLS
Autor/es:
ANDREANI V, CRESPO MI, MORÓN G, RIVERO V, MACCIONI M.
Lugar:
Viña del Mar, Chile
Reunión:
Congreso; Asociación Latinoamericana de Inmunología; 2009
Resumen:
Abstract
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Stimulation of melanoma B16 cells in vitro via
TLR4 inhibits subsequent tumor growth in
vivo in TLR4 deficient (TLR4lps-del) mice, indicating that TLR4 on tumor but
not on host cells is involved.
TLR4lps-del DCs stimulated with CpG in the presence of supernantant (SN)
from LPS-stimulated (SN B16+LPS) B16 cells overcome the inhibition of
activation observed when they are stimulated in the presence of non stimulated
B16-SN. Our goal was to further analyze the effect of SN B16+LPS on the
maturation of DC. We show that SN
B16+LPS increases IL6, TNFα, and IL12p70 produced by TLR4lps-del DCs, stimulated or not with CpG,
compared to the levels produced in the presence of B16-SN. CD11c+ cells from
spleen and lymph nodes of TLR4lps-del mice bearing tumors induced with B16 cells stimulated with LPS, showed higher percentage
of CD11c+ cells, expressing increased levels of CD40 marker (p<0.05) than
those observed in mice bearing tumors induced with non stimulated B16 cells . In
contrast, animals of this group show a reduced percentage Gr1+CD11b+ cells in
the spleen and lymph nodes of animals.
Therefore, stimulation of murine melanoma cells with LPS promotes an
improvement in the activation state of DCs in vitro and in vivo.