CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IDENTIFICATION OF iNKT FOLLICULAR HELPER CELLS AT EARLY STAGES OF TRYPANOSOMA CRUZI INFECTION
Autor/es:
GRUPPI, ADRIANA; BECCARIA, CRISTIAN G; MONTES, CAROLINA L.; ALMADA, LAURA; ACOSTA RODRIGUEZ E V; FIOCCA VERNENGO, FACUNDO
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias 2017; 2017
Institución organizadora:
SAI - SAIB - SAP - SAIC
Resumen:
(1239) IDENTIFICATION OF iNKT FOLLICULAR HELPERCELLS AT EARLY STAGES OF TRYPANOSOMA CRUZIINFECTIONLaura Almada, Facundo Fiocca Vernengo, Cristian G Beccaria,Carolina L Montes, Eva V Acosta-Rodriguez, AdrianaGruppiCentro de Investigaciones en Bioquímica Clínica e Inmunología(CIBICI)-CONICET, Facultad de Ciencias Químicas,Universidad Nacional de Córdoba, Córdoba, ArgentinaInvariant natural killer T cells (iNKT) are innate T cells wich expresssemi-invariant T cell receptors. They recognize both endogenousand exogenous lipid antigens presented by CD1d and theyrapidly produce cytokines following activation. It has been describedthat iNKT cells exert a variety of effector functions developing Th1,Th2 and Th17-like phenotype. Recent data showed that iNKT cellscould also acquire a Tfh-like phenotype, migrate into the GerminalCenter (GC) and sustain B cell responses. It is interesting to notethat an exquisite support of GC may actually represent a fundamentalmechanism to fine-tune the type and magnitude of antigen-specificantibody responses and that NKTfh could potentially representa new player in this landscape. The aim of our work was to evaluatethe presence and potential role of iNKT in GC response in T. cruziinfection. For that, C57BL/6 mice were infected with 5000 trypomastigotesof T. cruzi Tulahuen strain and the kinetic of iNKT and Tfhwas evaluated at different days post-infection (dpi) by flow cytometry.iNKT cells were identified by CD3int expression and bindingto α-GalCer-loaded CD1d tetramers. Tfh were characterized by theco-expression of CD3, CD4, ICOS, PD-1 and CXCR5. At 4dpi, weobserved that a substantial proportion of iNKT undergo Tfh-like phenotypedefined by the co-expression of ICOS, PD-1 and CXCR5,compared to uninfected controls (p