Chlamydia muridarum INDUCE THE EXPRESSION OF CO-INHIBITORY MOLECULES ON IMMUNE CELLS

DANIELA ANDREA PAIRA; CAROLINA OLIVERA; VIRGINIA ELENA RIVERO; FLORENCIA CELESTE SALAZAR ; RUBEN DARIO MOTRICH; GLORIA JANET GODOY

Buenos Aires

Congreso; REUNION CONJUNTA DE SOCIEDADES EN BIOCIENCIAS, BUENOS AIRES, NOVIEMBRE 2017; 2017

Sociedad Argentina de Inmunologia

Chlamydia trachomatis is the most commonly reported agent of sexually transmitted bacterial infections worldwide. This pathogen frequently leads to long-term persistent subclinical infections, both in male and female. This is in part due to certain mechanisms, not yet fully elucidated, that suppress the immune response to the pathogen. Recently, the role of co-inhibitory pathways in some chronic infections has been studied, and reports suggested that the overexpression of co-inhibitory molecules would be related to pathogen persistence. In the present work we aimed to evaluate the expression profile of co-inhibitory molecules using an ?in vitro? model with Chlamydia muridarum (Cm) stimulation. For that purpose, splenocytes from C57BL/6 and NOD mice were in vitro stimulated for 24-48 h with inactivated Chlamydia Elementary Bodies at different bacteria/cell ratios. After that, PD-L1 and PD1 expression was determined by flow citometry in different cell subpopulations. A significant and dose dependent increase in the percentage of PD-L1+CD20+ lymphocytes was found after Cm stimulation when compared with non-stimulated cells (P