Study of gut microbiota composition in lean and obese individuals from Cordoba, Argentina.

PORTELA, N; GALVÁN, C. A.; DEFAGÓ, M; RUIZ, S; COLL, J; PESOA, S; SALCEDO, R; SAKA, HA

Rochester

Conferencia; Mayo Clinic Individualizing Medicine Conference; 2017

Mayo Clinic

The worldwide obesity epidemic is stimulating efforts to identify hosts and environmental factors affecting energy balance. Gut microbiota has become a focus on research at the intersection of diet and metabolic health as it plays an essential role in host metabolism. Studies contrasting the gut microbiome from different countries have identified systematic differences in taxonomic microbial composition probably contributed by diet, geography, early life exposures to antibiotics and genetics of the host. No data on local gut microbiota composition is available in Argentina.AIM: The aim of this study is to determine the fecal microbiota composition of lean and obese Argentinean individuals, in order to generate local population data on gut microbial communities. These data could later be used to design strategies towards manipulating gut microbial communities as an approach to manage obesity.MATERIALS AND METHODS: Twenty individuals aged 21 to 55, were enrolled in this study and grouped as lean (BMI30, n=10) Total DNA was isolated from stool samples and 16S ribosomal RNA gene sequencing was performed on an Ion Torrent PGM using Ion 16S Metagenomics Kit following manufacturer protocols. Taxonomic assignment and Alfa and Beta diversity analysis was assessed using Ion Reporter workflow for Qiime SW. Statistical Analysis was carried out using Wilcoxon test.RESULTS: Our results showed substantial differences in the proportion of major phyla in lean and obese individuals. A reduction of Verrucomicrobia and Proteobacteria (p