Neutrophils activated by immunocomplexes modulate CD4+ cell response in lymph nodes.

CASTELL,S; HARMAN, MF; MORÓN, G; MALETTO, B; PISTORESI, MC

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017

Sociedades de Biociencia

(958) NEUTROPHILS ACTIVATED BY IMMUNOCOMPLEXESMODULATE CD4 T CELL RESPONSE IN LYMPHNODESSofía Daiana Castell, María Florencia, Gabriel Morón, BelkysMaletto, María Cristina PistoresiCIBICI-CONICET, Facultad de Ciencias Químicas, UNC,Córdoba, ArgentinaPreviously we demonstrated that immunocomplexes (IC) generatedby injecting OVA in the footpad of immunized mice that haveanti-OVA antibodies, induce the migration of OVA+ neutrophil todraining popliteal lymph nodes (D-poLNs). In the present study weevaluate the influence of neutrophils activated by IC on CD4 T cellresponse in lymph nodes.C57BL/6 mice were immunized with OVA emulsified in Freund?scomplete adjuvant and 15 days later boosted with OVA emulsified inFreund?s incomplete adjuvant. Ten days after last immunization theywere injected with OVA-FITC in the footpad and D-poLNs were obtained6 to 48 h later; saline solution was injected on footpad correspondingto control non-draining popliteal lymph nodes (ND-poLNs).OVA+ neutrophils migrated to D-poLNs 6 h after OVA injection(p