DEVELOPMENT OF INNATE T CELLS IN THE THYMUS UNDER INFECTIOUS/INFLAMMATORY SYSTEMIC CONDITIONS

CONSTANZA SAVID FRONTERA; MOTRAN, CLAUDIA C; FABIO CERBAN; RODRIGUEZ-GALAN, MARIA CECILIA; NATALIA BAEZ; VOLPINI, XIMENA

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC-SAI

Our previous work demonstrated that during the acute stage ofcertain infections with a strong Th1 component (Trypanosoma cruzior Candida albicans), SP CD8 thymocytes alter their differentiationfrom ?conventional? to ?innate? lineage. Innate CD8+ cells express aparticular phenotype (CD44hi CD122hi EOMEShi), produce high levelsof IFNg and have high cytotoxic activity. The switch in the SPCD8 lineage occurs by the inflammatory process since we obtainedsimilar data when we induced systemic expression of IL-12 and IL-18 by hydrodynamic injection of their cDNAs. The aim of our workis to determinate the stage at which innate CD8+ T cells developin the thymus and the molecular mechanisms that are involved inthis differentiation. Our data demonstrate that the innate phenotypegive rise as early as the double positive (DP) stage since when weco-cultured DP or SP CD8 CD45.1+ control thymocytes with CD45.2+T. cruzi-infected thymocytes we observed that CD45.1+ cells adoptthe mentioned innate features (p