C.albicans LIP in isolates from malignant lesions on in vitro model of human buccal cells

AGUILAR JJ; SOTOMAYOR CE; MIRÓ MS; ASCURRA A; CASTILLO G; RIERA F

Curitiba

Congreso; XV Forum on Fungal Infection in the Clinical Practice, INFOCUS 2017; 2017

INFOCUS

Candida is present in the normal oral flora, with albicans being the most frequent species. Since the 80´s, its association with other stomatological lesions has drawn significant attention. When immunocompetence breaks down, this opportunistic commensal fungus uses different virulence strategies to invade tissues, produce inflammatory response and cause damage. Among Candida´s virulence factors, hydrolytic enzymes such as protease (Sap) and lipase (LIP) have been described, but the contribution of lipase (LIP) to cell damage remains little known.ObjectiveOur aim was to evaluate the role as a virulence factor of C. albicans LIP released by isolates recovered from oral cancer lesion, in an in vitro model of human buccal cells.MethodsC. albicans strains isolated from lesions of oral squamous cell carcinoma (OSCC, n = 17) were co-cultured with human buccal mucosa fibroblasts in a Candida:fibroblast ratio 5:1 (3 x 105 fibroblasts /well) and incubated at 37°C during 24 h in a humid atmosphere and 5% CO2. C. albicans strains isolated from patients with healthy mucosa (HM, n = 15) were used as control. In order to establish the contribution of hydrolytic enzymes in cell damage, we added to the culture acetylsalicylic acid (ASA), a lipase inhibitor, or pepstatin (PEP), a Sap inhibitor. The evaluation of cell damage was performed using a damage index (DI = (LDH) sample / (LDH) monolayer). The release of cytokines IL-6 and TGF-β, after fungal stimulation, was evaluated by ELISA. Statistical analysis was performed by ANOVA test (p ≤0.05).ResultsC. albicansw was able to induce cell injury on buccal human fibroblast. The highest DI was observed in the isolates of OSCC, whereas ASA addition produced a significant decrease in damage (p = 0.0178 in HM; p = 0. 05 in OSCC). Interestingly, the addition of Sap (inhibitorof PEP) in the culturedid not significantly reduce the damage in co-cultures of the fungus isolated from OSCC and HM. A pro-inflammatory profile with a significant increase of IL-6 in the presence of both OSCC and HM isolates was observed (p < 0.0001 and 0.003, respectively), with higher levels when the cells were co-cultured with fungi recovered from malignant lesion (p= 0.01). In contrast, a significant decrease of the anti-inflammatory cytokine TGF-β was observed in the presence of the clinical isolates compared with the fibroblast monolayer basal production (p