Dectin-1 pathway-mediated immunomodulation in the local response during Candida albicans brain infection

MIRÓ MS; SOTOMAYOR CE; VIGEZZI C; ICELY PA; RODRÍGUEZ E; CEJAS H

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. LXV Reunión annual de la Sociedad Argentina de Inmunologia; 2017

Sociedades Cientificas Argentinas

-glucans of the fungal wall are recognized by Dectin-1 receptor. This interaction is essential for immune response to C.albicans in the periphery, but little is known about its role during Central Nervous System infections. -glucans are widely recognized as immunomodulators, and it has been proved in vitro that the activation of microglia through Dectin-1 attenuates the proinflammatory response. Our aim was to evaluate the role of Dectin-1 recognition in brain, during C.albicans systemic infection. Male C57BL/6 (WT) and Dectin-1 deficient (KO) mice were injected intravenously with C.albicans SC5314 (2.5x106 yeast), and at 4, 12, 24 and 48h post-infection (pi) the animals were sacrificed. The fungal burden (CFU) was studied in blood, kidney and brain homogenates. An early colonization was observed in the periphery as well as in brain(p4h