Type I interferons pathway has a relevant role during the course of vulvovaginal candidiasis

RODRÍGUEZ E; CEJAS H; MIRÓ MS; ICELY PA; VIGEZZI C; SOTOMAYOR CE

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. LXV Reunión annual de la Sociedad Argentina de Inmunologia; 2017

Sociedades Cientificas Argentinas

Type I Interferons (IFNs-I) constitute a cytokine family with diverse effects on immune cells. Recent studies have revealed that, besides viruses and bacteria, fungal pathogens can also induce IFNs-I production by professional APC. Candida albicans (Ca) is the principal agent of Vulvovaginal Candidiasis(VVC), a disease affecting 75% of women worldwide. We aimed to explore whether IFNs-I pathway modulates the immune response against Ca during VVC course. Female C57BL/6(WT) and IFNAR-/- (IFN-alpha receptor deficient) mice on estrus phase (estradiol-treated) were intravaginally inoculated with 5x106 Ca-SC5314 (Infected group) or PBS (Uninfected group) at day (D) 0. Untreated mice were used as controls.Vaginal lavage (VL) and vagina were obtained at D2, 4 and 8 pi. CFU studies revealed that both mice strains remained infected throughout the study period but no significant differences were observed in fungal burden. However, IFNAR-/- mice showed a significant increase on PMNs recruitment at D8 as compared to WT mice(p