CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Baculoviruses strongly potentiate OVA cytotoxic immune responses by capsid display
Autor/es:
PAULA MOLINARI; MARÍA I CRESPO; MARÍA J GRAVISACO; OSCAR TABOGA; GABRIEL MORÓN
Lugar:
Viña del Mar, Chile
Reunión:
Congreso; IX Congreso de la Asociación Latinoamericana de Inmunología; 2009
Institución organizadora:
Asoc. Latinoamericana de Inmunología
Resumen:
Baculoviruses are dsDNA viruses that are pathogenic for insects. They infect a broad range of mammalian cell types but do not replicate in these cells. The potential effects of these insect viruses on the immune responses of mammals are beginning to be understood. A recombinant baculovirus was designed which displays OVA on the capsid as a fusion with vp39 gene. We demonstrate in this study that baculoviruses have strong properties as adjuvants and as vectors for antigenic presentation in mice, allowing cross-presentation of the heterologous sequence transported and promoting potent CD4+, CD8+ and cytotoxic T cell adaptive responses against OVA. Baculoviruses also induce the in vitro and in vivo maturation of dendritic cells and the production of inflammatory cytokines, promoting adaptive immune responses. Interestingly, the OVA capsid display vector induces a strong cytotoxic T-cell response with minimal doses of antigen (corresponding to 30 ng of OVA protein) upon intravenous injection of BVOVA (5x107PFU). We also demonstrate that baculoviruses carrying the antigen are much more efficient in inducing a citotoxic T-cell response than baculoviruses co-administered with the antigen.