DENDRITIC CELLS ARE RESPONSIBLE FOR THE FAILURE IN CTLs GENERATION IN LSP1-DEFICIENT MICE

GROS, M; PISTORESI, MC; ACLAND,R; MALETTO, B; MORÓN, G; PASCUAL, M; AMIGORENA,S

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017

Sociedades de Biociencia

Leukocyte-specific protein 1 (LSP1) is an important regulator ofactin cytoskeleton remodeling, modulating leukocytes motility, dueto its F-actin binding domain. We have previously shown that Lsp1-/- mice have an impaired CTL response after antigen exposure.Moreover, Lsp1-/- dendritic cells (DCs) fail to induce a strong CTLresponse in vivo, migrate to lymphoid tissues, present antigens andproduce IL-12 when transferred into WT mice.In order to deepen the mechanisms operating this diminished CTLgeneration in Lsp1-/- mice, we first analyzed the ability of their CD8+T cells to proliferate and become activated. After in vitro stimulationwith αCD3/CD28, Lsp1-/- CD8+ T cells proliferated and up-regulatedCD25 as strongly as WT CD8+ T cells. Granzyme B production andIFNg release were slightly lower (p