CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PHOSPHOLIPIDS STUDIES IN HUMAN CORNEAL EPITHELIAL CELLS FROM CLIMATIC DROPLET KERATOPATHY PATIENTS.
Autor/es:
SUAREZ MARÍA F; A. URRETS-ZAVALIA, J.; ESPOSITO E; CORREA L; HM SERRA.
Reunión:
Congreso; ARVO ANNUAL MEETING 2017; 2017
Resumen:
Purpose: Climatic droplet keratopathy (CDK) is an acquireddegenerative disease of the cornea of unknown etiology, characterized by progressiveopalescence of cornea?s most anterior layers. CDK is highly prevalent incertain rural communities around the world living under harsh environmentalconditions (low humidity, constant winds and lack of protection to chronicexposure of ultraviolet radiation). To increase our knowledge about CDK?setiology, we performed a pilot study to evaluate phospholipids profile(phosphatidylcholine-PC- and phosphatidylserine-PS) in corneal epithelial cellsfrom 3 CDK patients.  Methods: Corneal epithelial cells from CDK areas (inter palpebral aperture) and from non affected areas (corneal epithelium covered by eye lids) were obtained from patients diagnosed with CDK whodid not presented any other surface eye disease. After reading a summary of the project, a consentform was signed by the patients.  Allprocedures were in accordance to a protocol approved by institutional reviewprocess and the tenets of the Declaration of Helsinki. Corneal cells weresubjected to lipid extraction using a modified Bligh and Dyer method; proteinconcentrations were determined using the Bradford?s method. Lipids wereidentified and subjected to ratiometric quantification using TSQ Quantum AccessMax triple quadrupole mass spectrometer, using appropriate class specific lipidstandards.  Results: We obtained lipid profiles for two classesof phospholipids: PC and PS. The phospholipid classes showed lower total amounts(pmol/µg protein) in affected areascompared to control areas from corneas of CDK patients: PC and PS control valueswere 5 times and 34 times higher than in CDK affected areas, respectively. Comparativeprofiles between CDK areas and control areas showed several common speciesbetween them. Unique lipids were also identified in the two classes ofphospholipids, in control areas that were absent in CDK areas and viceversa.   Conclusions: It was possible to quantify andidentify phospholipids in control and CDK affected areas in corneal epithelialcells from patients with CDK. The fact that we found lower amount of lipids inCDK areas compared to control areas could be attributed to the lipidperoxidation that takes place as a consequence of oxidative stress. Morestudies need to be done to confirm and extend these results.