Survival and Genes Expression of MRSA Epidemic Clones on an Environmental Inert Surface.

BARCUDI D.; SOLA C.; NICOLA J.P.; EGEA A.L.; BOCCO J.L.

Córdoba

Congreso; 52° Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular - SAIB 2016.; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Methicillin-resistantStaphylococcus aureus(MRSA) causes both hospital (HA) and community-acquired (CA) infections. In order to investigate the relationship between the epidemiological success of epidemic MRSA clones and their capacity to persist on an environmental inert surface, the survival and genes expression during the persistence on melamine surface were analyzed. Volumes of 100 μL of bacterial suspensions (1011?1012UFC/ml in saline solution, SS) of isolates representatives the epidemic clones (CAMRSA: ST5-IV, ST30-IV and USA300-ST8-IV, HAMRSA: ST5-I, ST5-IVnv, ST239-IIIA and ST5-II) were spotted onto a melamine surface. Dried MRSA were collected with SS at 24h; 10; 25 and 40 days to perform the colony counts and for RNA preparation. The genes expression (spaA, fnbpA, fnbpB, hla, psmA1A2, IsdD, agrA, RNAIII) was analyzed by qRT-PCR. All MRSA clones showed a similar survival tendency, decreasing progressively towards 40 day, being higher (p