IGF 1 MODULATES ZEB1 STABILITY DURING EPITHELIAL MESENCHYMAL TRANSITION

CABANILLAS ANA MARIA ; VAGLIENTI MARIA VICTORIA; LLORENS MARIA CANDELARIA

Córdoba

Congreso; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

ZEB1 is a transcription factor (TF) involved in EMT and oncogenesis. Previously we showed that an N-term fragment of ZEB1(NZEB1) was able to induce EMT by itself in mouse epithelial cells NMuMG. We intend to determine the mechanisms involved in regulating NZEB1 during EMT. NMuMG-NZEB1 cells were treated with specific inhibitors and activators of signaling pathways for 48h. Immunoblots against NZEB1, E-cadherin and α-tubulin (loading control) and the activity of pathways were assayed. None of the reagents modified the effect of NZEB1 on E-Cadherin expression. However, incubation with 5μM NVP (IGF1R inhibitor) for 48 h reduced NZEB1 expression (P