IL-17 and IFN-γ in the establishment of the liver protection after vaccination with kunitz type molecule during fasciola hepatica infection.

CELIAS DAINA; CHIODETTI ANA LAURA; ALLEMANDI DANIEL; MALETTO BELKYS A; MOTRAN C; CERVI LAURA; SILVANE LEONARDO M; SANCHEZ VALLECILLO MARÍA FERNANDA; PALMA SANTIAGO D

Buenos Aires, Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2016

Sociedad Argentina de Inmunología

The liver fluke Fasciola hepatica infect livestock worldwide and threaten foodsecurity. Fascioliasis is also a food borne disease with up to 17 million humansinfected. The prevailing control strategy based on anthelmintic drugs isunsustainable due to widespread resistance hence vaccination appears as anattractive option to pursue. In our laboratory we study a vaccine formulated byKunitz type molecule (KTM), an inhibitor of serine proteases with a key role insurvival of the parasite, and CpG-ODN/Coa-ASC16, an adjuvant with capacity forinduce response Th1 and Th17.The first results showed that the formulation KTM/CpG-ODN/Coa-ASC16 reduceliver damage caused by the infection in mice. What remains now is to evaluate howthis protection is developing.BALB/c mice were subcutaneously immunized with a solution of KTM/CpGODN/Coa-ASC16,CpG-ODN/Coa-ASC16 or saline buffer. Immunizations wereperformed at days 0, 7 and 14. Each mouse was immunized with an entire dose(250 µl) equally distributed at five sites: tail, back, the neck region and both hindlimbs (50 µl/site). CpG-ODN was administered at 35 mg/mouse/dose. The KTMdose injected was 10 mg/mouse/dose. One week after the last vaccination, micewere challenged orally with 6 F.hepatica metacercariae and euthanized 25 daysafter challenge.Splenocytes from KTM/CpG-ODN/Coa-ASC16 group restimulated with KTMsecrete significant amounts of IL-17 and IFN-γ (Student test, p