CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
“Complexity and function of cytokines response in Echinococcus granulosus experimental infection”.
Autor/es:
MOURGLIA-ETTLIN, GUSTAVO; BAZ, A; FRAGA, R; MERINO, MARIA CECILIA; AMEZCUA VESELY, MARIA CAROLINA; GRUPPI, ADRIANA; DEMATTEIS SILVIA
Lugar:
Rosario, Santa Fe. Argentina.
Reunión:
Congreso; VIII Congreso Argentino de Protozoología y Enfermedades Parasitarias; 2008
Institución organizadora:
Sociedad Argentina de Protozoología
Resumen:
Secondary hydatidosis in humans constitutes an important medical problem: it occurs by dissemination of protoscoleces after the accidental rupture of cysts and is due to the ability of protoscoleces to develop into new cysts. Experimental secondary hydatidosis by inoculation of protoscoleces in mice is the current model for studying the interactions between host´s immune system and the parasite. We have analyzed the cytokine response by splenocytes from infected mice at early stages of infection, and they showed a commitment to Th2 linage response. It is well known that major effectors functions of IFN-g (a prototypical Th1 cytokine) include macrophages activation. In this direction, we have determined that macrophages activated with IFN-g and LPS are able to kill protoscoleces in vitro through reactive nitrogen intermediates in a dose-dependent way. So, the suppression of macrophage activation via Th2 cytokines induction could be detrimental for the host favoring parasite survival. On the other hand, some studies support the existence of protoscoleces T-independent antigens. To further characterize those immunogenic glucidic moieties we have isolated a carbohydrate-rich fraction (E4+) using affinity chromatography with a monoclonal antibody specific for protoscoleces (E492/G1). That fraction induced IL-10 secretion by total peritoneal cells from both infected and naïve mice. Recently, we have found evidence that components within E4+ fraction can bind to CD19+ naïve cells. Then, we have performed a set of experiments aiming at elucidating if such a physical interaction with B cells derived in IL-10 secretion. The results indicated that total peritoneal B cells (B1 and B2 cells) from naïve mice, secreted IL-10 after E4+ in vitro stimulation, whereas purified peritoneal B1 cell produce IL-6 and TNF alpha, under the same conditions. Our results show that peritoneal B cells produce cytokines able to condition a Th2 response after E4 antigen interaction, suggesting a role of such antigen in conditioning cellular response during E. granulosus infection.