CONTRIBUTION OF TOMATO ELECTROPHILE COMPOUNDS TO THE THERMOTOLERANCE IN Caenorhabditis elegans

CARRANZA AV; ASIS RAMON; BONACCI G

Córdoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2016

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Tomato has been recognized to provide health benefits basedmostly on their antioxidant property. In this work, we proposedthat electrophile compounds could also contribute to thebeneficial effects of tomato. Therefore, we have evaluated theelectrophilic activity in tomato fruit with different colors, and theability of electrophilic extracts to confer thermal stress resistance (thermotolerance) in a model of stress adaptation inCaernohabditis elegans (C. elegans). To quantify theelectrophilic activity in tomato fruit, we optimized aspectrophotometer method using p-Nitrobenzenethiolassubstrate. Electrophilic activity and pigment profile was detectedand measured in chloroformic but not in aqueous-methanolicextracts. Correlation analysis showed a strong associationbetween electrophilic activity and β carotene content. Moreover, we have used the electrophile compound 1-Chloro-2,4-dinitrobenzeneas an approach to demonstrate electrophile-inducethermotolerance in C. elegans. In addition, chloroformextracts from different tomato fruit manifested diversethermotolerance activities that were positively associated withtheir electrophilic activity. All these results provide evidence that β carotene-derivedelectrophiles could be contributing to thebeneficial effects of tomato fruit through stress adaptation.