CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Testosterone favors a higher recruitment of neutrophils with reduced efficiency in killing bacteria
Autor/es:
LEIMGRUBER, C; MALDONADO, C; SCALERANDI, MV; NICOLA, JP; PEINETTI, N; QUINTAR, A
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión científica anual de la Sociedad Argentina de Investigación Clínica; 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Although androgens have been suggested to exert modulatory functions on adaptive immunity, there is scarce evidence about their role on the innate immune/inflammatory response. Considering that neutrophils are essential effector cells against bacterial pathogens, the aim of this work was to evaluate the effects of testosterone on neutrophils infiltrating the prostate gland. Adult male Wistar rats were first orchiectomized and immediately replaced with testosterone at physiological level (T) or vehicle (OX), and then subjected to acute prostatitis by intra-prostatic inoculation of E. coli (for 5 days, T+BP andOX+BP groups) or LPS (for 24 hs, T+LPS and OX+LPS groups). T+BP animals showed a higher neutrophil infiltration compared to OX+LPS, with intense E. coli immunostaining,correlating with the presence of phagocytosed bacteria in active neutrophils by electron microscopy. In LPS-induced prostatitis, testosterone treatment also promoted a higher neutrophil recruitment (Gr+) cells per gland by flow cytometry, which was correlated to anincreased mRNA expression of CXCL1 and CXCL2), with the cells having a lower myeloperoxidase (MPO) activity. Interestingly, sorted Gr+ infiltrating neutrophils showeda higher mRNA expression of IL10 and IL6 in T+LPS by qPCR. Finally, testosterone also increased thioglycollateinduced neutrophil recruitment in the peritoneum, withthe cells exhibiting a reduced bactericidal ability when coincubating ex vivo with E. coli. These findings reveal a intriguing role for testosterone on the early inflammatoryresponse in the prostate, with neutrophils being a main target. Testosterone increases local chemokine expression, leading to a higher recruitment of neutrophils to the site of infection. However, testosterone favors an IL10high MPOlow phenotype, with reduced efficiency in killing bacteria. This immunomodulatory effect of testosterone represents a novel factor to consider in alternative approches for inflammatory diseases.