Cell viability and Cell migration in Fibroblasts exposed to Ciprofloxacin-Polyelectrolytes combinations: Potential application in burn wounds

SANCHEZ MF; DONADIO AC; CRUZ DEL PUERTO M; OLIVERA ME; FLORES MARTIN J; GENTI RAIMONDI S

Rosario, Santa Fe

Congreso; 4ta Reunión Internacional de Ciencias Farmacéuticas; 2016

Colegio de Farmacéuticos y Universidades

Burns compromise the normal skin barrier functions. Ciprofloxacin (CIP) is a broad-spectrum antimicrobial agent, effective against burns pathogens. Besides, polyelectrolytes (PE) can benefit the burn healing process. The aim of this study was to evaluate in vitro different complexes CIP-PE in order to select the better materials to prepare dressings that could maintain optimal wound environment and avoid infections.For this purpose, we evaluated the cell viability, the cell migration and the proteins involved in cell migration in a human dermal primary fibroblasts culture in the presence/absence of different combinations of ciprofloxacin (CIP) with the following PE: sodium alginate (ALG), sodium hyaluronate (HIA) or carbomer (CB). By MTT assay, cell viability was evaluated incubating fibroblasts with CIP 0.0003% (low concentration, CIP(L)) or 0.003% (high concentration, CIP(H)) and PE 0.01% (PE(L)) or 0.1% (PE(H)). By wound healing assay, cell migration was evaluated determining the ability of the cells to move into an acellular space, using CIP(0.0075%) and PE(H). Additionally, 1-integrin, p-FAK and StarD7 proteins were evaluate, by western blot assay.Cell viability of all PE or CIP treatments was not modified, except for CIP(H) and CB(H) that decreased the cell viability in 59% and 65% respectively. Cell viability of CIP(L)-PE(L/H) combinations were similar that CIP(L) (94%), except by CIP(L)-CB(H) in 67%. All CIP(H)-PE(L/H) combinations increased the cell viability except for CIP(H)-CB(H) (65%), (p