POLÍMEROS MACROPOROSOS A PARTIR DE UN MONÓMERO DENDRÍTICO. ESTUDIO DEL EFECTO MULTIVALENTE EN LA INMOVILIZACIÓN DE METALES Y SU APLICACIÓN EN LA PURIFICACIÓN DE UNA PROTEÍNA

SANCHEZ MC; SERGIO D. G. SCHEJTMAN

Congreso; 16 SAM CONAMET; 2016

Dendritic structures are multi-branched molecules when its high density of functional groups improve multivalent interactions.[1, 2] In other hand, macroporous monoliths are used as base-supports in different separation techniques such chromatography due to its high porosity, reasonably low back pressure, etc.[3] Thus, were obtained hyper cross-linked dendronized polymers networks as macroporous monoliths, with excellent yields. This samples were synthetized using N-[Tris(hydroxymethyl)methyl]acrylamide (NAT), dendritic monomer from acrylate Behera?s amine (ABA) [4], and cross-linked with N,N′-Methylenebisacrylamide (BIS). Different reaction conditions were proved and the best were used (24 h at 70°C, DMSO and PEG6000/C14H29OH as porogenic mixture). The attachment of dendron was proved using IR spectroscopy and thermogravimetric analysis (TGA/DTA). Porous properties were studied by scanning electron microscopy and mercury intrusion porosimetry/nitrogen adsorption. Dendron changed chemistry properties and improved morphology and porosity. Immobilized metal ions such Cu (II) and Zn (II) were studied and demonstrated dendritic structure cause coordination with metal; when concentration of ABA increase from 20 to 30% w/w in polymer (50% of increasing), the amount of immobilized metal reach up 100% (0,13 to 0,26 mol Cu/mol of dendron) due to multivalent effect. Carried out alpha-2-Macroglobulin purifying with immobilized metal polymers and was shown its potential application such support in immobilized metal affinity chromatography (IMAC).