Candida albicans induces INF-b expression in female genital tract epithelial cells.

RODRIGUEZ E, VIGEZZI E, MIRÓ MS, GATTI G, ICELY PA, MACCIONI M, SOTOMAYOR CE

Mar del Plata

Congreso; LIX Reunión anual de la Sociedad Argentina de Investigación Clínica y LXIV reunión científica anual de la Sociedad Argentina de Inmunología (SAI); 2016

Type I interferons (IFNs-I) constitute a family of pleiotropic cytokinesbest known for their ability to induce an antiviral state and bycoordinating the immune cells involved in antiviral or antibacterialimmunity. IFNs-I are produced after virus or bacteria recognitionby TLRs and recent studies demonstrate that fungal recognitionthrough CTL receptors also induce activation of the IFNs-I in professionalAPC. Candida albicans (Ca) is the most common causeof vulvovaginal candidiasis (VVC) that affects approximately 75%of women worldwide. We aimed to study whether Ca recognitioninduces IFNs-I in epithelial cells of female genital tract in order toestablish a possible role during VVC. For this purpose, human cervicalepithelial cell line (HeLa) were stimulated with: Ca SC5314 strain(infective) (fungus: cell ratio 0.25:1, 0.5:1,1:1, 5:1), Ca treated withAmphotericin B (Ca-AMB) (non-infective) (5:1), Ca DNA complexedwith polyethylenimine (Ca DNA-PEI), Zymosan and Poly I:C for 24h.IFNb, IRF3, IRF7 and Mx1 mRNA levels were measured by qPCRand cytokine profile (IL1b, IL6, TNFa and TGFb) by ELISA. PolyI:C was able to induce a strong IFNb mRNA expression in HeLacells (p