Biological rhythms in the regulatory/tolerogenic functions of the immune activity in the gut

BARRIOS B., MACCIÓ-MARETTO L., NOVOTNY NUÑEZ I., CORREA S

Mar del Plata

Congreso; Reunión Anual Conjunta SAIC-SAI-SAFE; 2016

SAIC-SAI-SAFE

In the gut, the circadian clock orchestrates temporal organization of many aspects of physiology even immunity. As intestinal environment modulates the phenotype and function of immune cells, we wonder if over a 24h period there were variations in the activation or function of cells that could affect tolerance. We evaluated activation status of CD4+ and CD8+ lymphocytes and numbers of Foxp3+ regulatory T cells (Foxp3+ Treg) in mesenteric lymph nodes (MLN) from undisturbed C57BL/6 mice at 6, 12, 18 and 24h CT. We found that both CD4+ and CD8+ T cells bearing the markers CD62L+CD69+ oscillated in 24h period time with maximum at 6/18h and minimum at 12h CT (p