CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The microencapsulation of the flavonoid genistein with soluble chitosan allows efficient release in colon and attenuation of oxidative stress during experimental colitis.
Autor/es:
VANDEN BRABER NL, NOVOTNY NUÑEZ I, BOHL L, PORPORATTO C, MONTENEGRO M, CORREA SG
Lugar:
Mar del Plata
Reunión:
Congreso; Reunion Anual Conjunta SAIC-SAI-SAFE.; 2016
Institución organizadora:
SAIC-SAI-SAFE
Resumen:
Genistein (G) is a flavonoid with antioxidant, anti-inflammatory and anti-tumoral properties whose absorption occurs in the stomach and small intestine with significant loss of the bioactivity. The hypothesis of the present work was that upon microencapsulation, G could modulate the inflammatory process in the classic colitis model. Colitis was induced by administration of 3% (w / v) dextran sulfate sodium in drinking water for 5 days. G was microencapsulated (MCG) in water soluble chitosan (WSCh) by spray-drying. Female C57BL /6 mice were divided into 5 groups: Control, Colitis, Colitis+G, Colitis+MCG and Colitis+WSCh. G , MCG and WSCh were administered orally for 7 days starting at the beginning of colitis induction and animals were clinically evaluated during 10 days. The disease activity index (DAI) was calculated through weight loss, consistency and bleeding stool. Colon was isolated and processed for pro and anti-inflammatory cytokine analysis and superoxide dismutase (SOD), catalase and myeloperoxidase (MPO) activities. Statistical analyses were performed by ANOVA/Tukey. In MCG treated animals, the DAI was 70% lower than in Colitis group (p