Recruitment of immunocomplex-activated neutrophils modulate T cell response in lymph nodes.

MORÓN G; CASTELL,S; MALETTO, B; HARMAN, MF; PISTORESI, MC

Mar del Plata

Congreso; Sociedad Argentina de Inmunologia; 2016

Sociedad Argentina de Inmunología

Aprobado para ser presentado en la reunión Anual de la Sociedad Argentina de Inmunología. Mar del Plata. Noviembre 2016.RECRUITMENT OF IMMUNOCOMPLEX-ACTIVATED NEUTROPHILS MODULATE T CELL RESPONSE IN LYMPH NODESCastell, Sofía D1; Harman, María F1; Morón, Victor G1; Maletto, Belkys A1; Pistoresi, María C11CIBICI-CONICET- Facultad de Ciencias Químicas- UNC- Córdoba- Argentina Previously we demonstrated that immunocomplexes (IC) generated by injecting OVA in the footpad of immunized mice that have anti-OVA antibodies, induce neutrophils-OVA+ migration to draining popliteal lymph nodes (D-poLNs). In the present study we evaluate the influence of IC-activated neutrophils on T-cell response in lymph nodes.C57BL/6 mice were immunized with OVA emulsified in Freund?s complete adjuvant and 15 days later boosted with OVA emulsified in Freund?s incomplete adjuvant. Ten days after last immunization they were injected with OVA-FITC in the footpad and D-poLNs were obtained at different times (between 3 and 48 h); saline solution was injected on footpad corresponding to control non-draining popliteal lymph nodes (ND-poLNs). The different cell populations in the lymph nodes were characterized by flow cytometry.Three hours after footpad injection, an increased number of OVA-FITC+ Ly6Ghi neutrophils was observed in D-poLNs compared to ND-poLNs (p