LSP1 deficient mice have an impaired cytotoxic response after antigen exposure

RACHEL PAOLA ACLAND STRACK; ESTEFANÍA ZACCA; MERCEDES PASCUAL ; MARÍA CRISTINA PISTORESI; BELKYS MALETTO; GABRIEL MORÓN

Capital Federal

Congreso; LXIII Argentinean Immunology Society Meeting; 2015

Sociedad Argentina en Inmunología/ LASID

Background: The leukocyte-specific protein 1 (LSP1), isa 52-kDa cytoplasmic F-actin binding phosphoprotein expressedin all human and murine leukocytes as well as in endothelial cells.LSP1 is an important regulator of actin cytoskeleton remodeling,modulating neutrophil motility. No information is available aboutthe role of LSP1 in the triggering of immune response, particularlyin the biology of antigen presenting cells. We have previouslyreported that dendritic cells from Lsp1-/- mice fail to induce astrong CTL response upon transfer to wild-type mice. Methods:Lsp1-/- and wild-type mice (B6 background) were subcutaneouslyimmunized with OVA coupled to latex beads plus CpG-ODN asadjuvant into their footpads. Seven days later, popliteal lymphnodes (pLNs) were removed to evaluate T cell response. Results:the frequency and activation status of lymphoid and myeloid cellpopulations in pLNs of naïve Lsp1-/- vs wild-type mice aresimilar. After immunization, Lsp1-/- mice show a poorerexpansion of OVA-specific CD8+ T cells, as revealed by tetramerstaining (p