Skin immunity: IL-17 protects against epidermal dermatophytic infection and regulates the exacerbated inflammatory antifungal response.

BURSTEIN V.L.; THEUMER MG; GUASCONI L.; MENA C.; HERRERO M,; MASIH D.T.; CHIAPELLO L. S.

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinean Immunology Society Meeting II French Society for Immunology Meeting.; 2015

Sociedad Argentina de Inmunología

Microsporum canis is a zoophilic dermatophyte that causes contagious skin infections with mild to severe inflammatory lesions and is highly prevalent in immunocompetent children. Our previous data demonstrate that epicutaneous infection of C57BL/6 mice with M. canis is characterized by a Th17 response, however the in vivo role of IL-17 signaling has not been previously demonstrated. The aim of this study was to evaluate the impact of IL-17 in the outcome of skin infection and in the antifungal inflammatory response.Wild type (WT) and IL-17RA-/- (KO) C57BL/6 mice were infected with M. canis hyphae and at 4, 8, 18 and 45 days post-infection (d.p.i) histopathological analysis, skin fungal burden (HPLC ergosterol quantification) and extracutaneous fungal dissemination were determined. CD11b+ and T CD4+, T CD8+, Tγ and B cell populations and its cytokine production were analyzed (ELISA or intracellular staining and FACS) in skin cell suspensions or in skin draining lymph nodes cells (sdLN) after antigen specific-reestimulation. In addition, survival rate was registered.WT and KO mice resolved infection by 18 d.p.i., however KO mice showed severe inflammatory response in skin and sdLNs (p