CHARACTERIZATION AND FUNCTIONAL ANALYSIS OF REGULATORY T CELLS (Tregs) FROM MICE WITH DIFFERENTIAL SUSCEPTIBILITY TO AUTOIMMUNITY

GLORIA JANET GODOY; LEONARDO RODOLFO SANCHEZ; RUBEN DARÍO MOTRICH; VIRGINIA E. RIVERO

Buenos Aires

Congreso; LV LASID Meeting. LXIII Argentinean Immunology Society Meeting. II French-Argentinean Immunology Meeting; 2015

Sociedad Argentina de Inmunología

BackgroundWhile NOD mice are highly susceptible to develop spontaneous and induced autoimmunity, other strains are moderately prone (C57BL/6) or resistant (BALB/c). That high susceptibility has been related to defects in Tregs populations. Herein, we analyzed and compared the phenotype and functionality of Tregs from those different mice strains.MethodsSpleen and lymph node mononuclear cells (MNC) from NOD, C57BL/6 and BALB/c were obtained in steady state conditions (up to 8 weeks old). Tregs were stained (CD4+FoxP3+) and analyzed by FACS. CD4+CD25+ and CD4+CD25- MNC were sorted and activation and suppression assays were performed.ResultsSignificantly increased frequencies of CD4+CD25+Foxp3+, CD4+Foxp3+Helios+Nrp1+, and CD4+Foxp3+CCR6+ Tregs populations were observed in BALB/c mice (p