CHARACTERIZATION OF THE IFO MICE (TREG IFNAR-/-FOXP3GFP/GFP): AN INVALUABLE TOOL FOR STUDYING THE EFFECT OF TYPE I IFN (IFN-I) ON TREG BIOLOGY

PAULA ARAYA; EMILIANO ROSELLI; DAVID ANDRÉS NOCERA; NICOLAS NUÑEZ; LUCAS TRUCCO; MARIANA MACCIONI

Buenos Aires

Congreso; I Meeting LASID-FAIC-SAI; 2015

LASID-FAIC-SAI

Regulatory T cells (Treg) accumulate inside tumors: understanding which signals regulate their function is crucial. Here, we investigate the effect of type I IFN (IFN-I) on Treg biology. We developed a new strain of mice (IFO) by backcrossing Foxp3GFP females with IFNAR-/- males lacking the IFNAR1 subunit of the IFN-I receptor. After the fourth backcross cycle an homozygous strain was obtained. The leukocyte population in spleen, inguinal and mesenteric lymph nodes, thymus and blood in IFO animals showed a similar pattern to IFNAR-/- mice assessed by Flow cytometry, with reduced numbers of NK, CD4+, CD4+Foxp3+, CD8+ cells in spleen and MLNs compared to Foxp3GFP mice (p