CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Candida albicans pathogenic clinical strain upregulates relevant mediators involved in the Vulvovaginal Candidiasis immunopathogenesis
Autor/es:
MIRÓ, MARÍA SOLEDAD; RODRÍGUEZ, EMILSE; VIGEZZI, CECILIA; ICELY, PAULA ALEJANDRA; SOTOMAYOR, CLAUDIA ELENA
Lugar:
CABA
Reunión:
Congreso; IV LASID Meeting, LXIII Argentinean Immunology Society Meeting, II French-Argentinean Immunology Meeting; 2015
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Vulvovaginal candidiasis (VVC) is an infection that affects approximately 75% of women during their lifetime; 5% will develop the recurrent form (RVVC). The high incidence of RVVC might be attributable to powerful virulence factors of Candida albicans (Ca) or to host genetic factors. β-defensins (BDs) are antimicrobial peptides that play an important role in innate immunity. We aimed to evaluate the ability of Ca-RVVC clinical isolate compared with Ca collection strain to modulate immune mediators and recruitment of inflammatory cells in a VVC murine model. Female C57BL/6 mice were induced estrus phase by estradiol injections and intravaginally inoculated with 5.106 Ca-RVVC or Ca-ATCC36801 at day (D) 0. Not treated (NT) and estradiol treated mice were used as controls. At different D post infection (pi), vaginal lavage (VL) was obtained to evaluate fungal burden (CFU), cytokine levels (ELISA), BD1+ and infiltrating Gr-1+ cells (flow cytometry). At D2 and 8 pi fungal burden was higher during Ca-RVVC infection compared with Ca-ATCC (p