Genomic approach targeted to the genetic determinants of tomato fruit quality

KAMENETZKY, L1; ASÍS, R2; BASSI, S1, VAN SLUYS, MA3, FERNIE, AR4; ROSSI, M3, GIOVANNONI, J5,

Colonia, Alemania

Workshop; The 5th Solanaceas Genome Workshop; 2008

The adoption of tomato varieties selected for their high performance in terms of harvest index has considerably reduced accessibility to genetic basis of the Solanum genus. This fact has negatively impacted on the production of metabolites associated to the nutritional, flavor and pharmacological quality in tomato fruit. The main objective of our work is to determine the genetic determinants of key points regulating tomato fruit metabolism. In particular, this presentation conveys on the analysis of 5 regions of the Solanum pennellii genome spanning metabolic QTL previously mapped on an introgression line population (S. lycopersicum x S. pennellii). Two genomic libraries from S. pennellii were screened with 143 overgo designed on the same number of mapped markers. Two type of markers were used, the first based on information available on the Solanaceae Genome Network (www.sgn.cornell.edu) and the others were design on the bases of sequence information and further bioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.Solanum genus. This fact has negatively impacted on the production of metabolites associated to the nutritional, flavor and pharmacological quality in tomato fruit. The main objective of our work is to determine the genetic determinants of key points regulating tomato fruit metabolism. In particular, this presentation conveys on the analysis of 5 regions of the Solanum pennellii genome spanning metabolic QTL previously mapped on an introgression line population (S. lycopersicum x S. pennellii). Two genomic libraries from S. pennellii were screened with 143 overgo designed on the same number of mapped markers. Two type of markers were used, the first based on information available on the Solanaceae Genome Network (www.sgn.cornell.edu) and the others were design on the bases of sequence information and further bioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.Solanum pennellii genome spanning metabolic QTL previously mapped on an introgression line population (S. lycopersicum x S. pennellii). Two genomic libraries from S. pennellii were screened with 143 overgo designed on the same number of mapped markers. Two type of markers were used, the first based on information available on the Solanaceae Genome Network (www.sgn.cornell.edu) and the others were design on the bases of sequence information and further bioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.S. lycopersicum x S. pennellii). Two genomic libraries from S. pennellii were screened with 143 overgo designed on the same number of mapped markers. Two type of markers were used, the first based on information available on the Solanaceae Genome Network (www.sgn.cornell.edu) and the others were design on the bases of sequence information and further bioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.). Two genomic libraries from S. pennellii were screened with 143 overgo designed on the same number of mapped markers. Two type of markers were used, the first based on information available on the Solanaceae Genome Network (www.sgn.cornell.edu) and the others were design on the bases of sequence information and further bioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.www.sgn.cornell.edu) and the others were design on the bases of sequence information and further bioinformatic analyses of EST and BAC clones from S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.S. lycopersicum genome. End-sequence analyzes of 345 BAC/COS anchored clones including gene annotation; contigs assembling and comparison with the orthologous region of S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.S. lycopersicum were used to build physical maps of these regions. Fulllength sequences of selected clones containing candidate genes associated with the observed metabolic traits are being generated. The results obtained here allow the identification of wild alleles potentially involved in the tomato fruit quality determination.