Development of rapid strategies for testing of candidate Solanaceae QTLs

KAMENETZKY, LAURA (A) ASIS, RAMON (B) BERMUDEZ, LUISA (C) BAZZINI, ARIEL (A) ASURMENDI, SEBASTIAN (A) VAN SLUYS, MARIE-ANNE (C) GIOVANNONI, JIM (E) FERNIE, ALISDAIR R (D) ROSSI, MAGDALENA (C) CARRARI, FERNANDO (A)

Mexico, DF

Congreso; Plant Biolgy; 2008

American Society of plant biologists

The important mass of sequence information that is being released by the Tomato Genome Consortium (www.sgn.cornell.edu) is running in parallel with the availability of new tools developed in the field of plant functional genomics. Together with results from a genome-wide quantitative metabolic loci (QML) study of a tomato near isogenic lines (IL) population (derived from a cross between the cultivated tomato Solanum lycopersicum and the wild species Solanum pennellii) allow identification of key regulatory points on tomato metabolism. Alongside the dissection of previously identified QMLs for their spatial specificity, a systems-orientated view of the tomato metabolism is attempted to functionally evaluate genetic factors that regulate metabolism. This is being carried out through: QMLs physical mapping, candidate genes approaches, analysis of microRNAs (and their target genes mapping) and a combination of virus induced gene silencing (VIGS) and transgenesis methods. Five genomic regions were screened in details and 127 candidate genes were found. Moreover, a genomic survey for miRNA precursors rendered in a targeted database of putative regulatory regions. Results obtained here will be presented in the frame of the new quality standards that producers will must to face in coming years in regards the new mandatory conditions defined by the Regional Alimentary Code for 2010