LRP1 EXPRESSION IS DOWNREGULATED IN HUMAN AND MOUSE MONOCYTE SUBPOPULATIONS DURING ATHEROSCLEROTIC PROCESSES

ALBERTINI R; FERRER D; AMIGONE JL; COLLINO CJ; TINTI M; CAPRA RH; CASTRO C; CHIABRANDO GA

Amsterdam

Simposio; ISA 2015. 7th International Symposium on Atherosclerosis; 2015

IAS: International Atherosclerosis Society

LRP1 EXPRESSION IS DOWNREGULATED IN HUMAN AND MOUSE MONOCYTE SUBPOPULATIONS DURING ATHEROSCLEROTIC PROCESSESAbstract nr. 643Author Chiabrando , Gustavo, Facultad de Ciencias Quimicas Universidad Nacional de Cordoba, Cordoba, ArgentinaCo-author(s) - Albertini , RicardoCo-author(s) - Ferrer , DarioCo-author(s) - Amigone , JoseCo-author(s) - Collino , CesarCo-author(s) - Tinti , MariaCo-author(s) - Capra , RaulCo-author(s) - Castro , ClaudiaTopic The Pathogenesis of Atherosclerosis; Immunity, Inflammation, and the Endothelium Keywords Animal model,Atherosclerosis,Immunity,InflammationAtherosclerosis is an inflammatory process in which circulating monocytes are involved. Three monocyte subpopulations (MoSP) are characterized in human: classical, intermediate and nonclassical, whereas murine MoSP are basically LyC6hi and LyC6lo. The low-density lipoprotein receptor-related protein 1 (LRP1) is expressed in several tissues, included monocytes. LRP1 is involved in vascular remodeling and foam cell formation. However, LRP1 expression in MoSP in atherosclerosis is unclear. Herein we determine LRP1 levels in MoSP in patients with atherosclerotic lesions and in a murine model of atherosclerosis (apolipoprotein E deficient mice:KO-ApoE-/-) by flow cytometry (FC). Peripheral blood samples were obtained from: i) healthy individuals (HI group) (cholesterol40mg/dl (male); >50mg/dl (female), triglycerides