Study of the role of the cytokines of the IL-17 family in the CTL immunity against tumors.

RODRIGUEZ C; TOSELLO BOARI J; ARAUJO FURLÁN CL; CANALE F; GRUPPI A; MONTES CL; ACOSTA RODRIGUEZ EV

Congreso; I Meeting LASID-FAIC-SAI.; 2015

We demonstrated that during T. cruzi infection, intrinsic IL-17RA signaling mediates survival and proper activation of specific CD8+ T cells (CTLs), preventing early exhaustion. Because highly-functional CTL are critical for host-resistance to cancer, we evaluated the role of IL-17/IL-17R in modulating tumor-specific CTL immunity and tumor progression. For this, we used the melanoma model induced by injection of OVA-expressing B16-cells in WT mice. By real-time PCR we detected the IL-17RA-encoding transcripts in B16-cells, tumor, draining (D) and non-draining (ND) lymph nodes (LN) and spleen. Moreover, IL-17RC transcript was detected in B16-cells and tumor and IL-17F was detected in B16-cells, tumor and DLN. In contrast, IL-17A, IL-17C, and IL-17RE transcripts were low/undetectable in all samples. Next, we evaluated the specific and total CTL responses and tumor progression in WT, IL-17RA-knockout (KO) and IL-17A/F-KO-mice. We determined that tumor-bearing IL-17RA-KO-mice showed reduced numbers of total and tumor-specific CD8+ T cells (p