T cell subpopulations in nlrp3 inflammasome KO mice during the acute infection with Trypanosoma cruzi

PAROLI, AUGUSTO FABIÁN; GONZALEZ, PATRICIA VERÓNICA; GEA, SUSANA ELBA

Ciudad de Buenos Aires

Congreso; LXIII Reunión anual de la Sociedad Argentina de Inmunología; 2015

Sociedad Argentina de Inmunología

Background: NLRP3 inflammasome activation, as well as other innate receptors, plays a crucial role during T. cruzi infection. Previously, we have demonstrated an increase of IL-1beta-producing F4/80+ intra-hepatic leukocytes (IHL) associated with NLRP3 and TLR9 up-regulation. The aim of this work was to evaluate the involvement of NLRP3 on the expansion of CD4+, CD8+ T lymphocytes and F4/80+ IHL in addition to the production of reactive oxygen species (ROS) and nitric oxide (NO) by macrophages.Methods: Male C57BL/6 WT and nlrp3-/- mice were infected with T. cruzi (1000 i.p. injected-Tulahuen strain). The number of CD4+ and CD8+ T cells, IL1R, IL18R expression and intracellular cytokine production, as well as the detection of ROS and NO in F4/80+ IHL were evaluated at 14 and 21 dpi by flow cytometry.Results: Nlrp3-/- animals showed an increased F4/80+ IHL number (p