CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
T cell subpopulations in NLRP3 inflammasome KO mice during the acute infection with Trypanosoma cruzi
Autor/es:
PAROLI A; GONZALES PV; GEA S
Reunión:
Congreso; . Meeting LASID, FAIC, SAI 2015; 2015
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Background: NLRP3 inflammasome activation, as well as other innate receptors, plays a crucial role during T. cruzi infection. We have previously demonstrated an increase of IL-1β-producing F4/80+ intra-hepatic leukocytes (IHL) associated with NLRP3 and TLR9 upregulation. The aim of this work was to evaluate the involvement of NLRP3 on the expansion of CD4+, CD8+ T lymphocytes and F4/80+ IHL in addition to the production of reactive oxygen species (ROS) and nitric oxide (NO) by macrophages.Methods: Male C57BL/6 WT and nlrp3-/- mice were infected with T. cruzi (1000 i.p. injected-Tulahuen strain). The numbers of CD4+ and CD8+ T cells, IL1R, IL18R expression and intracellular cytokine production, as well as detection of ROS and NO in F4/80+ IHL were evaluated at 14 and 21 dpi by flow cytometry.Results: Nlrp3-/- animals showed an increased F4/80+ IHL number (p