CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CHLOROGENIC ACID BIOAVAILABILITY IN CAENORHABDITIS ELEGANS
Autor/es:
CARRANZA, A; SARAGUSTI, AC; ASIS, R
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión SAIB 2015; 2015
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Previously our workgroup has demonstrated that tomato extracts confer thermal stress resistance in C. elegans, where chlorogenic acid (CGA) was their main bioactive compound. The CGA activity might be explained by the effect of intact CGA or/and their biotransformed metabolites. In fact, we have identified four CGA metabolites by HPLC-MS in worms exposed to CGA active doses for 24h. Here, two metabolites (caffeic acid and ferulic acid), and the intact CGA, was evaluated in C. elegans. Adult worms were incubated with each compound for 18 h and then submitted to thermal stress conditions. Nematodes were fed with living and gentamicin-treated E. coli. Both metabolites showed the ability to rescue the worms from death by thermal stress; this activity was independent of the feeding conditions (live or dead bacteria). However, CGA was bioactive only in worms fed with live bacteria. To confirm these results, liquid bacteria (E. coli OP50) culture were fed with CGA. Dead bacteria fed with CGA and living bacteria culture were used as controls. The culture supernatants were used to supplement the NGM agar plates and nematodes were exposed to thermal stress. Only worms exposed to supernatant from living bacteria fed with CGA were rescued. These results evidence the relevance of the nematode?s gut microflora, and reveals that thermotolerance exerted by CGA is dependent on its biotransformation.