CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CORRELATION BETWEEN STARD7, b-CATENIN AND INOS TRANSCRIPT LEVELS IN RAT TISSUES
Autor/es:
FLORES-MARTÍN JÉSICA; RENA VIVIANA; PANZETTA-DUTARI GRACIELA; GENTI-RAIMONDI SUSANA
Lugar:
Carlos Paz
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2008
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.
Resumen:
StarD7 transcript encodes one of the 15 family members with
START domain present in the human genome. Highly similar
human StarD7 orthologous genes have been annotated in GenBank
databases in different genomes, suggesting a conserved
physiological function. We demonstrated that StarD7 gene
expression in human cells is regulated by Wnt/ß-catenin signaling
which is implicated in numerous aspects of development, cell
biology and physiology such as proliferation, inflammation, and
cancer. The aim of the present study was to evaluate StarD7
transcript expression in rat tissue samples and correlate them with ßcatenin
and inducible oxide nitric synthase (iNOS) ones, which is
also target of ß-catenin pathway. RT-PCR techniques for StarD7, ßcatenin
and iNOS were performed in liver, brain and ovary isolated
from normal rats. The transcripts were detected in all tissue samples
although the StarD7 tissue distribution was 2-4 times elevated in
brain compared to ovary and 60-80 times higher in liver than ovary.
These data show a significant correlation between expression of
StarD7 and ß-catenin mRNA level as well as with iNOS expression
and set more evidences indicating that StarD7 gene is a target of
Wnt/ß-catenin signaling pathway.
Supported by CONICET, FONCyT and SECyT-UNC.