CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CORRELATION BETWEEN STARD7, b-CATENIN AND INOS TRANSCRIPT LEVELS IN RAT TISSUES
Autor/es:
FLORES-MARTÍN JÉSICA; RENA VIVIANA; PANZETTA-DUTARI GRACIELA; GENTI-RAIMONDI SUSANA
Lugar:
Carlos Paz
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2008
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.
Resumen:
StarD7 transcript encodes one of the 15 family members with START domain present in the human genome. Highly similar human StarD7 orthologous genes have been annotated in GenBank databases in different genomes, suggesting a conserved physiological function. We demonstrated that StarD7 gene expression in human cells is regulated by Wnt/ß-catenin signaling which is implicated in numerous aspects of development, cell biology and physiology such as proliferation, inflammation, and cancer. The aim of the present study was to evaluate StarD7 transcript expression in rat tissue samples and correlate them with ßcatenin and inducible oxide nitric synthase (iNOS) ones, which is also target of ß-catenin pathway. RT-PCR techniques for StarD7, ßcatenin and iNOS were performed in liver, brain and ovary isolated from normal rats. The transcripts were detected in all tissue samples although the StarD7 tissue distribution was 2-4 times elevated in brain compared to ovary and 60-80 times higher in liver than ovary. These data show a significant correlation between expression of StarD7 and ß-catenin mRNA level as well as with iNOS expression and set more evidences indicating that StarD7 gene is a target of Wnt/ß-catenin signaling pathway. Supported by CONICET, FONCyT and SECyT-UNC.