alfa-MSH modulates LPS+IFN-ƒ× signaling in astrocytes in culture

GONZALEZ, P; CARUSO, C; SANCHEZ, M; CABANILLAS, ANA MARIA; LASAGA, M; SCIMONELLI, TERESA

Tandil, Bs.As. Argentina

Congreso; XL Reunion Anual SAFE; 2008

Sociedad Argentina de Farmacología Experimental

There is evidence that the melanocortin alpha-melanocyte stimulating hormone (a-MSH) has immunomodulatory and anti-inflammatory actions within the brain.The aim of the present study was to establish the effect of a-MSH on LPS+IFN-g-induced signalling pathways in hypothalamic neurones or astrocytes in culture.  We found that a-MSH suppresses LPS+IFN-g- induced nuclear translocation of the transcription factor NF-kB. Besides, the treatment with LPS+IFN-g stimulates phosphorylation of cAMP-responsive element-binding protein (CREB), but this effect was not modified by a-MSH. In neurones, LPS+IFN-g elicit a slightly increase in CREB activation. Treatment with a-MSH induced CREB phosphorylation and the combination of LPS+IFN-g and a-MSH induced an additive increase in CREB activation. Our results demonstrated that a-MSH can differently modulates signalling transduction pathways induced by LPS+IFN-g