Enhancement of immunological activity of CpG-ODN vehiculized in lyotropic liquid crystal carriers.

MALETTO, B; ALLEMANDI, D; MORÓN, G; PISTORESI-PALENCIA, MC; PALMA, S

Buenos Aires

Congreso; Reunión Anual de SAFE; 2008

SAFE

ENHANCEMENT OF IMMUNOLOGICAL ACTIVITY OF CPG-ODN VEHICULIZED IN LYOTROPIC LIQUID CRYSTAL CARRIERS Maletto B.(1), Alemandi D.A.(2), Morón G.(1), Pistoresi-Palencia M.C.(1), Palma S.D (2). Dep. de Bioquímica Clínica-CIBICI (1), Dep. de Farmacia (2), Fac. de Ciencias Químicas, U.N.C. Cdad. Universitaria, 5016 Córdoba, Argentina. CONICET sdpalma@fcq.unc.edu.ar CpG-ODN (oligonucleotides containing unmethylated CpG in particular base context) is a new adjuvant for vaccines that unfortunately has shown reduced bioavailability owed mainly to lack of chemical stability. In this work, we evaluated the adjuvant capacity of CpG-ODN incorporated into lamellar mesophases of lyotropic liquid crystals (coagels). For that, BALB/c mices were immunized (s.c.) three times during fifteen days (schedule: 0, 7 and 15) with a protein antigen (OVA, 60 ug/mice/dose) plus CpG-ODN (50ug/mice/dose) or OVA/CpG-ODN/coagel. On day 21, we measured specific antibodies in plasma and specific cytokines in cell culture supernatants (ELISA). The measured titles (log10) were:                                            IgG             IgG1          IgG2a OVA/CpG-ODN               3,9±0,0       3,4±0,1      2,7±0,2 OVA/CpG-ODN/coagel     5,1±0,0      4,4±0,1      3,8±0,2 The levels of IFN-g were higher in animals immunized with OVA/CpG-ODN/coagel than in those immunized with OVA/CpG-ODN (p<0,005).  These results indicate that CpG-ODN intensified the specific immune response when administered in coagels.