CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Syncytiotrophoblast differentiation is associated with transcriptional activation of genes belonging to the pregnancy-specific glycoprotein gene family
Autor/es:
CAMOLOTTO SOLEDAD; RACCA ANA; RENA VIVIANA; PATRITO LUIS; GENTI-RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA
Lugar:
Los Cocos, Córdoba
Reunión:
Simposio; III Latin-american Symposium on Maternal Fetal Interaction and Placenta: Basic & Clinical Research; 2007
Institución organizadora:
Grupo Latinoamericano de investigación en Placenta
Resumen:
The eleven human pregnancy-specific glycoprotein (PSG) genes are tightly linked within Ch 19q13.2. They are highly similar, have the same transcriptional orientation and are essential for maintenance of gestation. PSG mRNAs and proteins are markedly induced during trophoblast differentiation. However, little is known about the transcriptional control and expression pattern of each PSG gene. Objectives: This report examines the expression level and transcriptional activation of five PSG genes during in vitro syncytialization of human trophoblast cells. Methods: Normal villous cytotrophoblast and Jeg3 cells were induced to differentiate into syncytium- like structures. RNA isolated at different times was analyzed by semi-quantitative RT-PCR using PSG transcript specific primers. Transient transfection assays of PSG promoters:LUC constructs were performed. Results: PSG expression of all analyzed transcripts increased during differentiation. PSG3, 5 and 7 promoter constructs bearing up to 600 pb of the 5 ´ proximal regulatory region revealed different basal promoter activities which were increased upon differentiation. In addition, treatment of Jeg3 cells with chromatin modifying reagents induced increased transcription of endogenous PSG genes. Conclusions: Our results suggest that transcription of all PSG family members are activated during trophoblast differentiation. This activation involves regulatory elements present within the 5 ´ proximal regulatory region, as well as, chromatin modifications. Supported by CONICET, FONCYT and SECyT UNC.