Expression and localization of StarD7 in trophoblast cells

ANGELETTI SOFÍA; RENA VIVIANA; NORES RODRIGO; FRETES RICARDO; PANZETTA-DUTARI GRACIELA; GENTI-RAIMONDI SUSANA

Los Cocos, Córdoba

Simposio; III Latin-american Symposium on Maternal Fetal Interaction and Placenta: Basic & Clinical Research; 2007

Grupo Latinoamericano de investigación en Placenta

The StAR-related lipid transfer (START) domain is defined as a motif of around 200 amino acids implicated in lipid/sterol binding. In a previous study, we identified the StarD7 transcript that encodes one of the 15 family members with START domain present in the human genome. This transcript was found over-expressed in choriocarcinoma JEG- 3 cell line. In addition, we demonstrated that the recombinant StarD7 protein forms stable Gibbs and Langmuir monolayers at the air-buffer interface, showing marked surface activity and interaction with phospholipid monolayers, mainly with phosphatidylserine, cholesterol and phosphatidylglycerol. Objectives: This study was undertaken to evaluate the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: the expression and localization of StarD7 protein in trophoblastic samples. Methods: Objectives: This study was undertaken to evaluate the expression and localization of StarD7 protein in trophoblastic samples. Methods:Methods: Western blot, immunohistochemical and immunofluorescence assays were performed with two different antibodies obtained against over-expressed StarD7 and StarD7-C-terminal amino acids. Furthermore, StarD7 transcript levels were determined by quantitative real time PCR. Results: Western blot assays revealed a 34 kDa protein over-expressed in choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term normal placenta. Immunohistochemical data from early and full-term normal placenta showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore, an increase in StarD7 mRNA and protein expression as well as a partial subcellular protein re-localization was observed in in vitro differentiating cytotrophoblast cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC. cells isolated from full-term normal placenta. Conclusions: Our findings suggest that StarD7 may play a functional role in the process of trophoblast differentiation through phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.