Application of Partitioning Statistical Criteria to Establishment Reference Values in Lymphocyte Subset by Flow Cytometry

CÉSAR J. G. COLLINO; JAVIER R. JALDÍN FINCATI; SILVIA PERALTA; HUGO ROLAND; GUSTAVO A. CHIABRANDO

Budapest, Hungary

Congreso; ISAC (International Society for Analytical Cytology) XXIV International Congress; 2008

ISAC (International Society for Analytical Cytology)

The determination of Human Lymphocyte Subpopulations (HLS) by flow cytometry, included Lymphocytes CD3+ (TL), TLCD4+, TLCD8+ and TLCD4+/TLCD8+ ratio, allows to monitor patient infected with HIV, which are compared against reference values (RV). However, RV reported by the international bibliography are variable due to the absence of two main factors: analytical validation procedures and partitioning statistical criteria. In this work, using a validated method (ISO15189), we established RV for HLS in individuals with negative serology for HIV (397 females (F) and 279 male (M), age: 17-83 years) applying partitioning statistical criteria. The values of HLS were obtained by flow cytometry (Coulter Epics XL-MCL). The data were processed using partitioning statistical criteria based on Martin Gellerstedt’s algorithm (MGA). From the statistical analysis and MGA we determined the partition of the data in 6 subpopulations defined by age (interval of years) and gender (F and M): groups I and IV: 17-35 (F and M); groups II and V: 36-50 (F and M); and groups III and VI: >50 years (F and M). Hence, RV of total lymphocytes, TL, TLCD4+, TLCD8+ and TLCD4+/TLCD8+ ratio were defined. For each limit (lower and upper) of reference values, the 90% confidence interval was determined. In conclusion, this study allowed establishing RV for HLS in a validated method using a partitioning statistical criterion, which would allow increasing the assurance results and establishing an international criterion for reference values in HLS in different populations.