POOR CYTOTOXIC RESPONSE IN LEUKOCYTE SPECIFIC PROTEIN-1 DEFICIENT MICE IS ASSOCIATED WITH A REDUCED CROSS-PRESENTATION BY CD8a+ DCs

ACLAND, RACHEL; ZACCA, ESTEFANÍA; PISTORESI, MARÍA CRISTINA; MALETTO, BELKYS; MORÓN, GABRIEL

Mar del Plata

Congreso; LXII Reunión Científica de la Sociedad Argentina de Inmunología (SAI); 2014

The leukocyte-specific protein 1 (LSP1), is a 52-kDa cytoplasmic F-actin binding phosphoprotein expressed in leukocytes and endothelial cells that regulates cytoskeleton thus cell motility and polarization. It has been reported that human and murine LSP1-/- dendritic cells (DCs), infected with HIV show decreased proteasome degradation and altered intracellular trafficking of virus. This unexpected finding suggests a putative role of LSP1 in antigen cross-presentation in DCs. To investigate this hypothesis, we measured the ability of LSP1-/- DCs pulsed with an exogenous antigen (ovalbumin, OVA) plus polyuridilic acid (PU, a TLR7 ligand) to induce cytotoxic response. We found that wild-type (WT) mice that received the OVA/PU-pulsed LSP1-/- DCs had a lower CTL response (30±8% of specific lysis) compared to mice transferred with OVA/PU-pulsed WT DCs (64±3%, p